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antibody-techniques

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BOOK SUMMARY :

Read an overview of the methods and techniques you can use with antibodies includes ihc western blot flow cytometry and immunoprecipitationpublisher summary the normal function of the immune system is to provide protection against invasion by pathogens it is unfortunately also the source of less welcome events such as graft rejection and autoimmune diseases in which the system reacts against normal body constituentsthe applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniquesindirect fluorescent antibody techniques indirect fluorescent antibody ifa tests figure 2 are used to look for antibodies in patient serum for example an ifa test for the diagnosis of syphilis uses t pallidum cells isolated from a lab animal the bacteria cannot be grown on lab media and a smear prepared on a glass slidefluorescent antibody technique fat or immunofluorescence a technique used to show up the presence of a particular antigen in which an antibody is labelled with a fluorescent dye fluorochrome that combines with the antigen and is subsequently located by its fluorescence

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